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**Diagnosis and staging** are critical steps in managing triple-negative breast cancer, guiding treatment decisions and providing prognostic information. The diagnostic process typically begins with a clinical breast exam, followed by imaging studies such as mammography, ultrasound, and MRI. These imaging techniques help to identify suspicious lesions that may require further evaluation. A biopsy is then performed to obtain a tissue sample for pathological analysis. The biopsy sample is examined under a microscope to determine the presence of cancer cells and to assess the expression of ER, PR, and HER2. Immunohistochemistry (IHC) is used to determine the expression of these receptors. If the tumor cells do not express ER, PR, and HER2, the diagnosis of TNBC is confirmed. Once a diagnosis of TNBC is made, staging is performed to determine the extent of the cancer and whether it has spread to other parts of the body. Staging involves physical exams, imaging studies, and sometimes surgical procedures. The TNM staging system is commonly used to classify breast cancer based on the size of the tumor (T), the involvement of regional lymph nodes (N), and the presence of distant metastasis (M). The stage of the cancer is a critical factor in determining the appropriate treatment strategy. Early-stage TNBC, such as stage I and II, may be treated with surgery, radiation therapy, and chemotherapy. Advanced-stage TNBC, such as stage III and IV, may require more aggressive treatment approaches, including chemotherapy, targeted therapy, and immunotherapy. *Accurate diagnosis and staging are essential for developing an effective treatment plan and improving outcomes for patients with TNBC.* In addition to traditional staging methods, molecular profiling can provide additional information about the characteristics of the tumor and may help to guide treatment decisions. Gene expression assays, such as Oncotype DX and MammaPrint, can assess the risk of recurrence and predict the likelihood of benefit from chemotherapy. These assays can be particularly useful in patients with early-stage TNBC to help determine whether chemotherapy is necessary. Furthermore, circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) can be detected in the blood and may provide information about the presence of residual disease and the response to treatment. These liquid biopsies are being investigated as potential tools for monitoring TNBC and guiding treatment decisions.
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